Virodex™: Superior replacements to Triton™ X-100

Are you utilising viral inactivation and cell lysis techniques in your bioprocessing applications?

We’re introducing our new range of bioprocessing detergents specifically designed to support the key steps in your biomanufacturing processes.

The Virodex™ range for viral inactivation and cell lysis is REACH-compliant, compendial, sustainable, and has been optimised for use in biomanufacturing of recombinant proteins, blood fractionation products, viral vectors, and more.

We maintain the highest level of safety and manufacturing standards, to ensure our offerings are cGMP EXCiPACT manufactured, compliant to the corresponding Pharmacopeial monographs, and much more.

Virodex™ TXR-1

Virodex™ TXR-1 is a readily biodegradable, nonionic surfactant for viral inactivation and cell lysis, meeting the needs of biopharmaceutical applications as an effective and safe replacement for the...

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Virodex™ TXR-2

Virodex™ TXR-2 is a 40% biobased, nonionic surfactant for viral inactivation and cell lysis, meeting the needs of biopharmaceutical applications as an effective and safe replacement for the...

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Virodex™: Safe and effective viral inactivation and cell lysis

Sustainable, REACH-compliant, and cGMP EXCiPACT manufactured

Compendial grade, with known parenteral applications*

No risk of nitrosamine formation

Equivalent performance to Triton™ X-100, with better performance than other replacements on the market

Offered in a range of pack sizes, including single-use

And more...

Introducing Virodex™: Superior replacements to Triton™ X-100

FEATURED WEBINAR

Triton™ X-100, a detergent often utilized in biomanufacturing, has been banned (with exemptions) in Europe effective January 2021 because of its harmful environmental impact. To address this issue, we have identified two sustainable alternatives that are compendial-grade, cGMP EXCiPACT-manufactured, REACH-compliant materials. This presentation will highlight that these alternatives exhibit equivalent performance to Triton™ X-100 for viral inactivation and cell lysis and outperform three market alternatives for viral inactivation.

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What is the Virodex™ range of detergents?

The Virodex™ range offers two products, TXR-1, and TXR-2. Both products have been shown to have equivalent or better performance in virus inactivation and cell lysis applications than Triton™ X-100 and three other marketed products available today. The Virodex™ products are non-denaturing detergents that show excellent protein compatibility and high batch-to-batch consistency. These qualities make Virodex™ TXR-1 or TXR-2 excellent options to consider incorporating into your existing or new biomanufacturing processes.

Virodex™: performance equivalent to Triton™ X-100:

To assess the performance of our product range, we compared its performance to other virus inactivation products available on the market today and Triton™ X-100, in multiple tests relevant to biomanufacturing processes:

Viral inactivation - potency and kinetics
Cell lysis of CHO-K1 and HEK293T cells
Protein compatibility.

Findings show that Virodex™ TXR-1 and TXR-2 had equivalent performance to Triton™ X-100 in all comparative tests and performed better than the other marketed products in virus inactivation tests.

Additional COA testing is included in our specifications: bacterial endotoxin, Total Aerobic Microbial Count (TAMC), and Total Yeast and Mould Count (TYMC) to support use in sterile manufacture.

Learn more about how Virodex™ has been featured in the media:

Choose Virodex™ as your Triton™ X-100 replacement

Despite Triton™ X-100 being a widely used detergent for viral inactivation and cell lysis in biopharma, the substance group it belongs to (phenol ethoxylates) was added to the Candidate List of Substances of Very High Concern (SVHC) of the REACH (Registration, Evaluation and Restriction of Chemicals) Regulation in 2012 by the European Chemicals Agency1.

With a sunset date of January 4th 2021, its use and sale in the EU was banned, except for those with an exemption certificate². The time for applying for exemptions has now passed.

For those who have previously relied on Triton™ X-100 as a surfactant or detergent in their manufacturing processes there is now an urgent need to find a suitable replacement.

Our Virodex™ range offers two solutions: TXR-1, and TXR-2.

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow.

1. Candidate list of substances of very high concern for authorisation (2012) ECHA. Available at: https://echa.europa.eu/candidate-list-table/-/dislist/details/0b0236e1807db570 (Accessed: 19 May 2023).

2. Annex XIV Authorisation list (2022) ECHA. Available at: https://www.echa.europa.eu/authorisation-list (Accessed: 19 May 2023).

*Virodex™ TXR-1 chemistry has known parenteral applications

Hear directly from our experts...

Watch our expert Dr. Kritika Bajaj discuss our product range in more detail and learn how Virodex™ could be the solution to your replacement requirement.

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From Triton X-100 to Virodex: exploring our innovative solution to the Triton X-100 ban

For those who have previously relied on Triton™ X-100 as a surfactant or detergent in their manufacturing processes there is now an urgent need to find a suitable replacement. Explore Croda's innovative solution to your formulation challenges - Virodex!

Concerns about Triton™ X-100 have been an ongoing discussion across multiple regulatory authorities around the world

Triton™ X-100, a non-ionic surfactant with a hydrophilic polyethylene oxide chain and a hydrophobic aromatic group1, has found widespread use in the biopharmaceutical industry with applications including virus inactivation1, cell lysis and membrane permeation2. Even though it is used heavily throughout the industry, the degradation products from Triton™ X-100 exhibit long-lasting estrogenic properties in fish, birds, and mammals, causing disruptions to their endocrine systems, raising concerns in the bioprocessing industry.